Use of Molecular Beacon Probes for Real-Time PCR Detection of Plasmodium falciparum and Other Plasmodium Species in Peripheral Blood Specimens

molecular beacon probes-base multiplex nasba real-time for detection of hiv-1 and hcv.

developed in 1991, nucleic acid sequence-based amplification (nasba) has been introduced as a rapid molecular diagnostic technique, where it has been shown to give quicker results than pcr, and it can also be more sensitive. this paper describes the development of a molecular beacon-based multiplex nasba assay for simultaneous detection of hiv-1 and hcv in plasma samples.a well-conserved region...

Simplified Pan-species Real-time PCR-based Detection of Plasmodium Spp. in Blood Smear

BACKGROUND We aimed to quicken and simplify the detection of Plasmodium in blood samples by developing and testing a pan-Plasmodium real-time PCR for accurate screening of individuals suspected of malaria. METHODS A single primer/probe set for pan-species Plasmodium-specific real time PCR targeting a conserved region of the small subunit 18S ribosomal DNA was designed and evaluated for rapid ...

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

Evaluation of anti-plasmodium effect of Iranian snake venom by Real-time PCR

Introduction: Malaria is one of the most important parasitic diseases and one of the important health issues especially in tropical and subtropical areas of the world. . The importance of this disease is due to its high prevalence, significant mortality, as well as drug resistance and, side effects of current drugs in treatment. Venom is a complex mixture of active pharmaceutical ingredients. ...

RESISTANCE OF PLASMODIUM FALCIPARUM TO CHLOROQUINE IN SOUTH EASTERN IRAN

In vivo and in vitro assessments of the response of P. falciparum to chloroquine using WHO standard kits and techniques were carried out in I ran Shahr, Sistan and Baluchestan province of Iran in 1985. In the in vivo assessment, 24 malaria patients treated with chloroquine (25mg/kg over three days) were followed up for one to four weeks. The mean parasite clearance time was 4.3 days and in...